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Index > Protein center > Nos3(Gene name) > Rat
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  • Nos3 (Gene name),
  • Nitric oxide synthase, endothelial (Protein name ),  NOS3_RAT from NCBI database.
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  • General Annotation
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  • Antigen Annotation
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  • 3D
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  • Predicted Eptitope
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  • Vaild Sequence
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  • Related Databases
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  • Citations  (6)
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  • Gene name:
    Nos3;
    Protein name:
    Nitric oxide synthase, endothelial;
    Alternative:
    NOS type III(NOSIII);Endothelial NOS(eNOS);Constitutive NOS(cNOS);EC-NOS;
    Organism:
    Rat (Rattus norvegicus). 
    General Annotation
    Sub Unit:
    Homodimer. Interacts with NOSIP and NOSTRIN.
    Function:
    Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
    Subcellular Location:
    Membrane caveola Cytoplasm cytoskeleton Golgi apparatus Cell membrane Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle, which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
    Protein Attributes:
    Sequence length:
    1202
    Sequence:
    50:
    MGNLKSVGQE | PGPPCGLGLG | LGLGLCGKQG | PASPAPEPSQ | APVPPSPTRP | 
    100:
    APDHSPPLTR | PPDGPKFPRV | KNWEVGSITY | DTLSAQAQQD | GPCTPRRCLG | 
    150:
    SLVFPRKLQS | RPTQGPSPTE | QLLGQARDFI | NQYYNSIKRS | GSQAHEQRLQ | 
    200:
    EVEAEVVATG | TYQLRESELV | FGAKQAWRNA | PRCVGRIQWG | KLQVFDARDC | 
    250:
    RTAQEMFTYI | CNHIKYATNR | GNLRSAITVF | PQRYAGRGDF | RIWNSQLVRY | 
    300:
    AGYRQQDGSV | RGDPANVEIT | ELCIQHGWTP | GNGRFDVLPL | LLQAPDEPPE | 
    350:
    LFTLPPELVL | EVPLEHPTLE | WFAALGLRWY | ALPAVSNMLL | EIGGLEFPAA | 
    400:
    PFSGWYMSSE | IGMRDLCDPH | RYNILEDVAV | CMDLDTRTTS | SLWKDKAAVE | 
    450:
    INVAVLYSYQ | LAKVTIVDHH | AATASFMKHL | ENEQKARGGC | PADWAWIVPP | 
    500:
    ISGSLTPVFH | QEMVNYFLSP | AFRYQPDPWK | GSAAKGTGIT | RKKTFKEVAN | 
    550:
    AVKISASLMG | TVMAKRVKAT | ILYGSETGRA | QSYAQQLGRL | FRKAFDPRVL | 
    600:
    CMDEYDVVSL | EHEALVLVVT | STFGNGDPPE | NGESFAAALM | EMSGPYNSSP | 
    650:
    RPEQHKSYKI | RFNSVSCSDP | LVSSWRRKRK | ESSNTDSAGA | LGTLRFCVFG | 
    700:
    LGSRAYPHFC | AFARAVDTRL | EELGGERLLQ | LGQGDELCGQ | EEAFRGWAQA | 
    750:
    AFQAACETFC | VGEDAKAAAR | DIFSPKRSWK | RQRYRLSTQA | ESLQLLPRLT | 
    800:
    HVHRRKMFQA | TILSVENLQS | SKSTRATILV | RLDTGSQEGL | QYQPGDHIGV | 
    850:
    CPPNRPGLVE | ALLSRVEDPP | PSTEPVAVEQ | LEKGSPGGPP | PGWVRDPRLP | 
    900:
    PCTLRQALTY | FLDITSPPSP | RLLRLLSTLA | EESSEQQELE | ALSQDPRRYE | 
    950:
    EWKWFRCPTL | LEVLEQFPSV | ALPAPLILTQ | LPLLQPRYYS | VSSAPSAHPG | 
    1000:
    EIHLTVAVLA | YRTQDGLGPL | HYGVCSTWMS | QLKAGDPVPC | FIRGAPSFRL | 
    1050:
    PPDPNLPCIL | VGPGTGIAPF | RGFWQDRLHD | IEIKGLQPAP | MTLVFGCRCS | 
    1100:
    QLDHLYRDEV | LDAQQRGVFG | QVLTAFSRDP | GSPKTYVQDL | LRTELAAEVH | 
    1150:
    RVLCLEQGHM | FVCGDVTMAT | SVLQTVQRIL | ATEGSMELDE | AGDVIGVLRD | 
    1200:
    QQRYHEDIFG | LTLRTQEVTS | RIRTQSFSLQ | ERQLRGAVPW | SFDPPTQETP | 
    1202:
    GS
    3D Structure:
    N/A
    Predicted Eptitope:
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    EIAab Sequence  Vaild Sequence:
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    Related Databases
    Pfam:
    String:
    KEGG:
    UniGene:
    SMR:
    Uniprot:
     
    FOR
    ELISA Kit for Rat Nitric oxide synthase, endothelial
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    E0815r
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    96T
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    ELISA Kit for Rat Nitric oxide synthase, endothelial
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    E0815h
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    96T
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    ELISA Kit for Rat Nitric oxide synthase, endothelial
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    E0815d
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    96T
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    ELISA Kit for Rat Nitric oxide synthase, endothelial
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    E0815p
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    ELISA Kit for Rat Nitric oxide synthase, endothelial
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    E0815m
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    96T
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    ELISA Kit for Rat Nitric oxide synthase, endothelial
    Cat.:
    E0815b
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    MSDS:
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    Packing:
    96T
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    CLIA Kit for Rat Nitric oxide synthase, endothelial
    Cat.:
    U0815h
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat Nitric oxide synthase, endothelial
    Cat.:
    U0815r
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Range:
    Please sign in first.
    CLIA Kit for Rat Nitric oxide synthase, endothelial
    Cat.:
    U0815d
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Nitric oxide synthase, endothelial
    Cat.:
    U0815p
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Nitric oxide synthase, endothelial
    Cat.:
    U0815m
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    CLIA Kit for Rat Nitric oxide synthase, endothelial
    Cat.:
    U0815b
    Price:
    Please sign in first.
    MSDS:
    Please sign in first.
    Packing:
    96T
    Polyclonal Antibody for Rat Nitric oxide synthase, endothelial
    Cat.:
    P0815Rb-h
    Price:
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    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Nitric oxide synthase, endothelial
    Cat.:
    P0815Rb-r
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Nitric oxide synthase, endothelial
    Polyclonal Antibody for Rat Nitric oxide synthase, endothelial
    Cat.:
    P0815Rb-m
    Price:
    Please sign in first.
    Packing:
    40ug/0.2ml
    Polyclonal Antibody for Rat Nitric oxide synthase, endothelial
    Polyclonal Antibody for Rat Nitric oxide synthase, endothelial
    Monoclonal Antibody for Rat Nitric oxide synthase, endothelial
    Monoclonal Antibody for Rat Nitric oxide synthase, endothelial
    Monoclonal Antibody for Rat Nitric oxide synthase, endothelial
    Monoclonal Antibody for Rat Nitric oxide synthase, endothelial
    Monoclonal Antibody for Rat Nitric oxide synthase, endothelial
    Monoclonal Antibody for Rat Nitric oxide synthase, endothelial
    Protein for Rat Nitric oxide synthase, endothelial
    Protein for Rat Nitric oxide synthase, endothelial
    Protein for Rat Nitric oxide synthase, endothelial
    Protein for Rat Nitric oxide synthase, endothelial
    Protein for Rat Nitric oxide synthase, endothelial
    Protein for Rat Nitric oxide synthase, endothelial

    R&D Technical Data
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    s
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    Precision
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    Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

    Intra-Assay CV: ≤3.9%

    Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

    Inter-Assay CV: ≤7.7%

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    Recovery
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    Recovery was determined by spiking various levels of ACE into serum and plasma .

    Sample Type

    Average(%)

    Recovery Range(%)

    Serum

    99

    91-103

    Plasma

    98

    93-106

     

     

     

     

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    Linearity
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    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.  

     

    Sample

    1:2

    1:4

    1:8

    1:16

    serum(n=5)

    92-105%

    90-101%

    95-105%

    92-109%

    Saliva(n=3)

    89-95%

    93105%

    89-94%

    90-106%

    heparin plasma(n=5)

    93-104%

     

    94-110%

    101-111%

    91-110%

     

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    References
    1. 1.
      "NIDD, a novel DHHC-containing protein, targets neuronal nitric-oxide synthase (nNOS) to the synaptic membrane through a PDZ-dependent interaction and regulates nNOS activity."
      Saitoh F. , Tian Q.B. , Okano A. , Sakagami H. , Kondo H. , Suzuki T.
      J. Biol. Chem.279:29461-29468(2004) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA]
    2. 2.
      "Downregulation of endothelial nitric oxide synthase (NOS III) in rat aorta following in-vivo hypoxia."
      Toporsian M. , Govindaraju K. , Nagi M. , Eidelman D. , Thibault G. , Ward M.E.
      Submitted (1998-08) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-288
      strain: Sprague-Dawley.
    3. 3.
      "Increased resistance to myocardial ischemia in the Brown Norway vs. Dahl S rat: role of nitric oxide synthase and Hsp90."
      Shi Y. , Hutchins W. , Ogawa H. , Chang C.-C. , Pritchard K.A. Jr. , Zhang C. , Khampang P. , Lazar J. , Jacob H.J. , Rafiee P. , Baker J.E.
      J. Mol. Cell. Cardiol.38:625-635(2005) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 14-1202
      strain: SS/JrHsdMcwi.
      tissue: Heart.
    4. 4.
      "Cloning and expression of the rat endothelial nitric oxide synthase."
      Seidel B. , Jiang L. , Wolf G.
      Submitted (1998-09) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 473-672 AND 951-1192
      tissue: Brain.
    5. 5.
      "Differential expression and induction of mRNAs encoding two inducible nitric oxide synthases in rat kidney."
      Mohaupt M.G. , Elzie J.L. , Ahn K.Y. , Clapp W.L. , Wilcox C.S. , Kone B.C.
      Kidney Int.46:653-665(1994) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 633-862
      strain: Sprague-Dawley.
    6. 6.
      "Endothelin-1, endothelin receptors and ecNOS mRNA expression in vital organs during traumatic shock in rats."
      Minchenko A.G. , Armstead V.E. , Opentanova I.L. , Lefer A.M.
      Submitted (1998-09) to the EMBL/GenBank/DDBJ databases
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1015-1132
      strain: Sprague-Dawley.
      tissue: Lung.
    7. 7.
      "Constitutive endothelial nitric oxide synthase gene expression is regulated during lung development."
      Kawai N. , Bloch D.B. , Filippov G. , Rabkina D. , Suen H.C. , Losty P.D. , Janssens S.P. , Zapol W.M. , de la Monte S. , Bloch K.D.
      Am. J. Physiol.268:L589-L595(1995) [PubMed] [Europe PMC] [Abstract]
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1018-1080
      strain: Sprague-Dawley.
      tissue: Lung.
    8. 8.
      [15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: PHOSPHORYLATION AT THR-494 AND SER-1176
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